133kDa核孔蛋白(NUP133)多克隆抗體 

• 來源多抗制備
• 宿主兔
• 效價-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 133kDa核孔蛋白(NUP133)多克隆抗體標記物無標記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應用WB, ICC, IHC-P, IHC-F, ELISA
• 133kDa核孔蛋白(NUP133)多克隆抗體Polyclonal Antibody to Nucleoporin 133kDa (NUP133)
  Organism Species: Mus musculus (Mouse)
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
  9th Edition (Revised in Jul, 2013)
  [ PRODUCT INFORMATION ]
  Immunogen: NUP133, Mouse
  Clonality: Polyclonal
  Host: Rabbit
  Immunoglobulin Type: IgG
  Purification: Affinity Chromatography.
  Applications: WB, ICC, IHC-P, IHC-F, ELISA
  Concentration: 200μg/mL
  UOM: 100μg
  133kDa核孔蛋白(NUP133)多克隆抗體[ IMMUNOGEN INFORMATION ]
  Immunogen: Recombinant NUP133 (Leu36~His240) expressed in E.coli. Accession No.: RPB152Mu01
  Sequence: The target protein is fused with two N-terminal Tags, His-tag and
  T7-tag and its sequence is listed below.
  MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF - LSLGS AVNSPVLFSP
  AGRRSSVSSR GTPTRIFPHH SISESVNYDV RVFGSSLPVK IMEALTMAEA DEQLSVHVDE
  GGWACLVCTE KLLIWKIAVS PVTKLSVCKE LQLPPSDFHG SADLVALSYA ATSGEVHSVQ
  AVSVMVATKE GSIRYWPSLA REDTYSDTCV DLGGEKMCRF LTAVQGGSFI LSSVGSQLVR
  LIPESSGKIH
  [ ANTIBODY SPECIFITY ]
  The antibody is a rabbit polyclonal antibody raised against NUP133. It has been
  selected for its ability to recognize NUP133 in immunohistochemical staining and
  western blotting.
  133kDa核孔蛋白(NUP133)多克隆抗體[ APPLICATIONS ]
  Western blotting: 1:100-400
  Immunocytochemistry in formalin fixed cells: 1:100-500
  Immunohistochemistry in formalin fixed frozen section: 1:100-500
  Immunohistochemistry in paraffin section: 1:50-200
  Enzyme-linked Immunosorbent Assay: 1:100-200
  Optimal working dilutions must be determined by end user.
  [ IMAGES ]
  [ CONTENTS ]
  Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  [ QUALITY CONTROL ]
  Content: The quality control contains recombinant NUP133 (Leu36~His240)
  disposed in loading buffer.
  Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
  5uL pe r well when used in enhan ced chemilumes cent (ECL ).
  Note: The quality control is specifically manufactured as the positive control.
  Not used for other purposes.
  Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
  Store at 4
  oC for frequent use. Stored at -20oC to -80
  oC in a manual defrost freezer
  for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  Used in DAB staining on fromalin fixed
  paraffin-embedded Heart tissue
  Used in Western Blot, Sample:
  Recombinant NUP133, Mouse