2A5細胞

ATCC Number： CRL-2444?

生長狀態： 懸浮生長

是否是腫瘤細胞： 0

物種來源： 小鼠

數量： 大量

細胞形態： **樣

**類型： IgM; kappa light chain

運輸方式： 凍存運輸

Designations: 2A5

2A5細胞Depositors: PJ Bjorkman

Isotype: IgM; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products:2A5細胞 immunoglobulin; monoclonal antibody; against HFE

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Comments: The cell line was established by Jose A. Lebron and Pamela J. Bjorkman in 1997.

Animals were tolerized with human beta2-microglobulin and immunized with purified, soluble, human HFE.

Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]).

The antibody recognizes the HFE portion of the HFE/beta2 heterodimer.

HFE is a major histocompatibility complex (MHC) related protein that is mutated in the iron-overload disease hereditary hemochromatosis.

2A5細胞This antibody can be used for ELISA applications and to immunoprecipitate soluble HFE; it is not reactive in Western Blot assays.

Propagation: ATCC complete growth medium: HL-1 medium supplemented with 4 mM L-glutamine, 1 mM sodium pyruvate and 1% fetal bovine serum. HL-1 medium can be obtained from from Lonza (catalog number 77201).

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.

Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Attached cells may be subcultured by tapping the sides of the flask until cells are dispersed.

Preservation: HL-1 Complete Serum-free Medium, 75%; fetal bovine serum, 20%; DMSO, 5%.

Related Products: recommended serum:ATCC 30-2020

References: 39791: Lebron JA, et al. 2A5細胞Tolerization of ***** mice to immunodominant proteins before monoclonal antibody production. J. Immunol. Methods 222: 59-63, 1999. PubMed: 10022372

39792: Lebron JA, et al. Crystal structure of the hemochromatosis protein HFE and characterization of its interaction with transferrin receptor. Cell 93: 111-123, 1998. PubMed: 9546397