THB-5 [HB-5, HB5]細(xì)胞
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
運(yùn)輸方式： 凍存運(yùn)輸
生長狀態(tài)： 懸浮生長
細(xì)胞形態(tài)： **樣
**類型： IgG2a; kappa light chain
ATCC Number： HB-135?
Designations: THB-5 [HB-5, HB5]
Depositors: TF Tedder
Isotype: IgG2a; kappa light chain
THB-5 [HB-5, HB5]細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: THB-5 [HB-5, HB5]細(xì)胞immunoglobulin; monoclonal antibody; against human C3d receptor (CR2, Epstein-Barr virus (EBV) receptor); against CD21
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Comments: Animals were immunized with the SB human B cell line.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with the human C3d receptor (14500 dalton). This receptor also is the receptor for Epstein-Barr virus (EBV) on human B cells.
THB-5 [HB-5, HB5]細(xì)胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium, 85%; fetal bovine serum, 15%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 990: Weis JJ, et al. Identification of a 145,000 Mr membrane protein as the C3d receptor (CR2) of human B lymphocytes. Proc. Natl. Acad. Sci. USA 81: 881-885, 1984. THB-5 [HB-5, HB5]細(xì)胞PubMed: 6230668
