G14D細(xì)胞

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞類型： 其他細(xì)胞類型

是否是腫瘤細(xì)胞： 0

物種來源： 其他

器官來源： 外周血

生長狀態(tài)： 懸浮生長，多細(xì)胞聚集

品系： Marion

G14D細(xì)胞ATCC Number： CRL-2760?

Designations: G14D

Depositors: NW Miller

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, multicell aggregates

Organism: Ictalurus punctatus

Morphology: lymphoblast

Source: Strain: Marion

Organ: peripheral blood

Cell Type: G14D細(xì)胞lymphocyte T lymphocyte;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1998

Receptors: T cell antigen receptor, expressed

Cytogenetic Analysis: diploid

Comments: The G14D cell line developed in 1998 is an immortal homozygous T cell line spontaneously derived by in vitro lipopolysaccharide (LPS) stimulation of peripheral blood from a normal gynogenetic channel catfish.. The cells were, subsequently, cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. The cells constitutively produce interleukin-like factors.

Propagation:G14D細(xì)胞 ATCC complete growth medium: A 1:1 mixture of Leibovitz's L-15 medium with 2 mM L-glutamine and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 10% double distilled water and 5% heat-inactivated catfish serum

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 27.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml. Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Fetal Bovine Serum supplented with 7.5% DMSO

Storage temperature: liquid nitrogen vapor phase

References: 61263: Hogan RJ, et al. G14D細(xì)胞Molecular and Immunologic Characterization of Gynogenetic Channel Catfish (Ictalurus punctatus). Mar. Biotechnol. 1: 317-327, 1999. PubMed: 10489406