132C4A/4細(xì)胞
細(xì)胞形態(tài)： **樣
**類型： IgG1
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
ATCC Number： HB-8086?
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
Designations: 132C4A/4
132C4A/4細(xì)胞Depositors: Johns Hopkins University
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 132C4A/4細(xì)胞immunoglobulin; monoclonal antibody; against surface (cercariae) glycoprotein on Schistosoma mansoni
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with cercariae glycoprotein from Schistosoma mansoni.
132C4A/4細(xì)胞Spleen cells were fused with P3X63Ag8 myeloma cells.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.2 units/ml insulin, 1 mM sodium pyruvate, 0.15 mg/ml oxaloacetic acid, 0.01 M HEPES and HAT, 70%; NCTC 135 medium, 10%; heat- inactivated fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium, 40%; fetal bovine serum, 50%; DMSO, 10%
Related Products: Recommended medium 132C4A/4細(xì)胞(without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 3511: Strand M. Diagnosis and treatment of fluke infections with monoclonal antibodies. US Patent 4,416,866 dated Nov 22 1983
