13762 MAT B III細胞

生長狀態： 懸浮生長

運輸方式： 凍存運輸

品系： Fischer 344 (F344)

器官來源： 乳腺

是否是腫瘤細胞： 0

物種來源： 大鼠

ATCC Number： CRL-1666?

相關**： 腺癌

數量： 大量

細胞形態： 上皮樣

13762 MAT B III細胞Designations: 13762 MAT B III

Depositors: AE Bogden

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension or loosely adherent

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: epithelial

Source: Organ: mammary gland

Strain: Fischer 344 (F344)

Disease: adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 13762 MAT B III細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: The 13762 MAT B III cell line was established at the EG&G Mason Research Institute from a transplantable rat ascites tumor derived from the 13762 solid mammary adenocarcinoma.

Prior to its deposit, the cell line was tested and found negative for the following viruses:

Pneumonia virus of mice (PVM), reovirus type 3 (Reo3), K virus, polyomavirus, Sendai virus, GDVII (mouse poliovirus), Kilham rat virus (KRV), minute virus of mice (MVM), SV5, H-1 virus and ectromelia virus.

The line grows as small clusters of rounded cells lightly attached to the substrate, or as a static suspension.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: 13762 MAT B III細胞Protocol: Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at 1 X 10 exp5/ml and 1 X 10 exp6 cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007

recommended serum:ATCC 30-2020

References: 32293: Xing RH, et al. Prevention of breast cancer growth, invasion, and metastasis by antiestrogen tamoxifen alone or in combination with urokinase inhibitor B-428. Cancer Res. 57: 3585-3593, 1997. 13762 MAT B III細胞PubMed: 9270032