LL 29 (AnHa)細胞

數量： 大量

細胞形態： 成纖維樣

生長狀態： 貼壁生長

年限： 26 years

ATCC Number： CCL-134?

相關**： 其他**

細胞類型： 成纖維細胞

LL 29 (AnHa)細胞是否是腫瘤細胞： 0

物種來源： 人

運輸方式： 凍存運輸

器官來源： 肺

Designations: LL 29 (AnHa)

Depositors: RG Crystal

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: LL 29 (AnHa)細胞Homo sapiens

Morphology: fibroblast

Source: Organ: lung

Disease: idiopathic pulmonary fibrosis

Cell Type: fibroblast

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Age: 26 years

LL 29 (AnHa)細胞Gender: female

Ethnicity: Caucasian

Propagation: ATCC complete growth medium: Ham's F12K medium, 85%; fetal bovine serum, 15%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium and add fresh 0.25% trypsin, 0.03% EDTA for 1 to 2 minutes. Remove trypsin, EDTA and incubate the culture at 37C for 10 to 20 minutes with a small volume of trypsin - EDTA. LL 29 (AnHa)細胞Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%