1H11細(xì)胞
ATCC Number： HB-10611?
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
細(xì)胞形態(tài)： **樣
**類(lèi)型： IgG1
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
1H11細(xì)胞物種來(lái)源： 小鼠
Designations: 1H11
Depositors: Washington Research Foundation
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: 1H11細(xì)胞Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against bone type 1 collagen amino terminal telopeptide
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Comments: Animals were immunized with a conjugate of keyhole limpet hemocyanin (KLH) and a telopeptide having a 3-hydroxypyridinium crosslink derived from the amino terminal domain of bone type I collagen.
Spleen cells were fused with mouse myeloma cells.
The antibody can be used to detect and quantitate degradation products of bone type I collagen in body fluids.
Propagation: 1H11細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22055: Eyre DR. 1H11細(xì)胞Hybridoma cell line producing an antibody to type-I collagen amino-terminal telopeptide. US Patent 5,300,434 dated Apr 5 1994
