BxPC-3細胞

器官來源： 胰腺

年限： 61 years

數(shù)量： 大量

ATCC Number： CRL-1687?

相關(guān)**： 腺癌

運輸方式： 凍存運輸

細胞形態(tài)： 上皮樣

生長狀態(tài)： 貼壁生長

是否是腫瘤細胞： 1

物種來源： 人

BxPC-3細胞Designations: BxPC-3

Depositors: MH Tan

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: pancreas

Disease: adenocarcinoma

Cellular Products: mucin; pancreas cancer specific antigen (pancreas cancer associated antigen); carcinoembryonic antigen (CEA)

Permits/Forms: BxPC-3細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 13

D13S317: 11

D16S539: 9,11

D5S818: 11

D7S820: 10,13

THO1: 9

TPOX: 8

vWA: 14,18

Age: 61 years

BxPC-3細胞Gender: female

Comments: The cells do not express the cystic fibrosis transmembrane conductance regulator (CFTR).

A CFTR positive pancreatic line, Capan-1 is available (ATCC HTB-79).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 22737: BxPC-3細胞Loor R, et al. Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567-578, 1982. PubMed: 6754226

23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903

23153: Chambers JA, Harris A. Expression of the cystic fibrosis gene and the major pancreatic mucin gene, MUC1, in human ductal epithelial cells. J. Cell Sci. 105: 417-422, 1993. PubMed: 7691840

58045: Tan MH, et al. Characterization of a new primary human pancreatic tumor line. Cancer Invest. 4: 15-23, 1986. PubMed: 3754176