SK-UT-1B細胞

生長狀態(tài)： 貼壁生長

是否是腫瘤細胞： 1

物種來源： 人

ATCC Number： HTB-115?

相關**： 其他**

細胞形態(tài)： 上皮樣

年限： grade III

器官來源： **

運輸方式： 凍存運輸

數(shù)量： 大量

組織來源： endometrium

Designations: SK-UT-1B

Depositors: G Trempe

SK-UT-1B細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: uterus

Tissue: endometrium

Tumor Stage: grade III

Disease: mesodermal tumor (mixed); consistent with leiomyosarcoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) SK-UT-1B細胞The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.

Tumorigenic: Yes

Antigen Expression: Blood type B; Rh+

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 10,13

D16S539: 12,14

D5S818: 10,11

D7S820: 9,10

THO1: 7

TPOX: 8

vWA: 16

Cytogenetic Analysis: (P27) hypodiploid to diploid

SK-UT-1B細胞Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1-2

PGM1, 1

PGM3, 1

Age: 75 years

Gender: female

Ethnicity: Caucasian

Comments: This is a substrain of SK-UT-1 (see ATCC HTB-114) which reportedly differs in morphology, karyology and in the type of tumor which develops on animal inoculation.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended

Medium Renewal: SK-UT-1B細胞Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22259: Chen TR. SK-UT-1B, a human tumorigenic diploid cell line. Cancer Genet. Cytogenet. 33: 77-81, 1988. PubMed: 3383166

22414: Chen TR. SK-UT-1B cell line has the diploid karyotype. A reply. Cancer Genet. Cytogenet. 48: 139-141, 1990. PubMed: 2372783

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080