PA-1細胞
運輸方式： 凍存運輸
年限： 12 years
數(shù)量： 大量
是否是腫瘤細胞： 1
物種來源： 人
ATCC Number： CRL-1572?
相關(guān)**： 其他**
細胞形態(tài)： 上皮樣
生長狀態(tài)： 貼壁生長
器官來源： 卵巢
Designations: PA-1
PA-1細胞Depositors: BC Giovanella
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: ovary
Disease: teratocarcinoma
Derived from metastatic site: ascites
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: The cells form tightly knit colonies, and differentiate to form embryoid bodies when cultured in low serum concentration, or at low plating densities or when treated with 5-bromo-2'-deoxyuridine.
The embryonic antigen PCC4 is expressed, but F9 is not detectable.
Tumorigenic: PA-1細胞Yes
Oncogene: N-ras + (activated)
Antigen Expression: HLA A28, B12
DNA Profile (STR): Amelogenin: X
CSF1PO: 9,12
D13S317: 9,10
D16S539: 9,12
D5S818: 11
D7S820: 9
THO1: 7,9
TPOX: 11
vWA: 15,17
Isoenzymes: G6PD, B
Age: 12 years
Gender: female
Ethnicity: Caucasian
Comments: PA-1細胞The line was established from cells taken from ascitic fluid.
The cells form tightly knit colonies, and differentiate to form embryoid bodies when cultured in low serum concentration, or at low plating densities or when treated with 5-bromo-2'-deoxyuridine.
The embryonic antigen PCC4 is expressed, but F9 is not detectable.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
References: 22179: . . In Vitro 10: 382, 1974.
22368: Zeuthen J, et al. Characterization of a human ovarian teratocarcinoma-derived cell line. Int. J. Cancer 25: 19-32, 1980. PubMed: 6931103
22530: Giovanella BC, et al. Heterotransplantation of human malignant tumors in "nude" thymusless mice. II. Malignant tumors induced by injection of cell cultures derived from human solid tumors. J. Natl. Cancer Inst. 52: 921-930, 1974. PubMed: 4524119
22950: Tainsky MA, et al. PA-1細胞An activated rasN gene: detected in late but not early passage human PA1 teratocarcinoma cells. Science 225: 643-645, 1984. PubMed: 6740333
23112: McGowan-Jordan IJ, et al. Suppression of tumorigenicity in human teratocarcinoma cell line PA-1 by introduction of chromosome 4. Cancer Res. 54: 2568-2572, 1994. PubMed: 8168081
