HuNS1細胞

數(shù)量： 大量

生長狀態(tài)： 懸浮生長

ATCC Number： CRL-8644?

相關(guān)**： 其他**

細胞形態(tài)： **樣

運輸方式： 凍存運輸

細胞類型： **母細胞

是否是腫瘤細胞： 1

物種來源： 人

Designations: HuNS1

Depositors: Medical College of Wisconsin Research Foundation, Inc.

HuNS1細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Disease: myeloma

Cell Type: lymphoblast

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DNA Profile (STR): Amelogenin: X,Y

HuNS1細胞CSF1PO: 10,12

D13S317: 12

D16S539: 11

D5S818: 10,12

D7S820: 10

THO1: 9,9.3

TPOX: 8

vWA: 16,19

Gender: male

Comments: HuNS1細胞This line was derived from the GM4672 cell line (Human Genetic Mutant Cell Repository, Coriell Institute for Medical Research) following treatment with ethylmethanesulfonate (EMS).

The cells are HAT sensitive, do not secrete immunoglobulin and are negative for Epstein-Barr virus.

The line is suitable for use as a fusion partner for human B cells.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine, 4.5g/L glucose, 10 mM HEPES, 1 mM sodium pyruvate, 1.5 g/L sodium bicarbonate, 0.02 mM 2-amino-6-methylmercaptopurine, 85%; fetal bovine serum, 15%

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Freeze medium: Complete Culture Medium, 95%; DMSO, 5%.

Storage temperature: liquid nitrogen vapor phase

References: 3864: Winkelhake JL. HuNS1細胞Myelomas for producing human/human hybridomas. US Patent 4,720,459 dated Jan 19 1988