Hs 936.T(C1)細胞

ATCC Number： CRL-7687?

相關**： 黑色素瘤

運輸方式： 凍存運輸

數量： 大量

器官來源： 皮膚

細胞形態： 上皮樣

生長狀態： 貼壁生長

是否是腫瘤細胞： 1

Hs 936.T(C1)細胞物種來源： 人

Designations: Hs 936.T(C1)

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: skin

Disease: melanoma

Hs 936.T(C1)細胞Cellular Products: melanin

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Gender: male

Comments: Clone of ATCC CRL-7686.

Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.

Please see the NBL Repository description.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Hs 936.T(C1)細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: 1:2 to 1:4

Medium Renewal: every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO 5%

Storage temperature:Hs 936.T(C1)細胞 liquid nitrogen vapor

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020