BC-2細(xì)胞
組織來源： pleural effusion
生長狀態(tài)： 懸浮生長
年限： 31 years *****
細(xì)胞類型： B**細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來源： 人
ATCC Number： CRL-2231?
相關(guān)**： **瘤
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
BC-2細(xì)胞Designations: BC-2
Depositors: Cornell Res. Fndn., Inc.
Biosafety Level: 2 [Cells contain EBV and KSHV (HHV-8) viral genomes ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Tissue: pleural effusion
Disease: lymphoma
Cell Type: B lymphocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. BC-2細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Roche Transfection Reagents)
Antigen Expression: CD45 +; EMA + (epithelial membrane antigen)
Age: 31 years *****
Gender: male
Ethnicity: Caucasian
Comments: BC-2 is a lymphoma cell line originated in 1992 by Ethel Cesarman and Giorgio Inghirami in the laboratory of Daniel M. Knowles.
The cells were derived from a pleural cavity based lymphomatous effusion.
The cells contain two viral genomes: Epstein-Barr virus (EBV) and Kaposi sarcoma associated herpesvirus (KSHV, provisionally designed HHV-8).
KSHV is a recently identified and largely uncharacterized virus.
BC-2細(xì)胞The BC-2 cell line allows in vitro culture of KSHV viral genomes.
It is also a practical source of KSHV DNA to be used as a positive control for screening tests.
Propagation: ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 2 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Doubling Time: 48 to 72 hrs
References: 22572: Moore PS, et al. Primary characterization of a herpesvirus agent associated with Kaposi's sarcoma. J. Virol. 70: 549-558, 1996. PubMed: 8523568
23358: Cesarman E, et al. In vitro establishment and characterization of two acquired immunodeficiency syndrome-related lymphoma cell lines (BC-1 and BC-2) containing Kaposi's sarcoma-associated herpesvirus-like (KSHV) DNA sequences. Blood 86: 2708-2714, 1995. PubMed: 7670109
23395: Chadburn A, et al. CD30 (Ki-1) positive anaplastic large cell lymphomas in individuals infected with the human immunodeficiency virus. Cancer 72: 3078-3090, 1993. BC-2細(xì)胞PubMed: 8221575
23493: Cesarman E, et al. Kaposi's sarcoma-associated herpesvirus-like DNA sequences in AIDS- related body-cavity-based lymphomas. N. Engl. J. Med. 332: 1186-1191, 1995. PubMed: 7700311
39036: Cesarman E, et al. KSHV positive cell lines. US Patent 5,908,773 dated Jun 1 1999
