Hs 729細胞
生長狀態(tài)： 貼壁生長
ATCC Number： HTB-153?
數(shù)量： 大量
相關(guān)**： 橫紋肌肉瘤
年限： 74 years
細胞形態(tài)： 成纖維樣
組織來源： muscle
運輸方式： 凍存運輸
是否是腫瘤細胞： 1
物種來源： 人
Hs 729細胞Designations: Hs 729 [Hs 729T]
Depositors: RB Owens
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Tissue: muscle
Disease: rhabdomyosarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Hs 729細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,12
D16S539: 11
D5S818: 11,12
D7S820: 8,9
THO1: 6,9.3
TPOX: 11
vWA: 16,17
Isoenzymes: G6PD, B
Age: 74 years
Gender: male
Ethnicity: Caucasian
Hs 729細胞Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
Preservation: Culture medium, 95%; Hs 729細胞DMSO, 5%
