SW 900細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
年限： grade IV
器官來(lái)源： 肺
細(xì)胞形態(tài)： 上皮樣
ATCC Number： HTB-59?
相關(guān)**： 鱗狀細(xì)胞癌
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
數(shù)量： 大量
Designations: SW 900 [SW-900, SW900]
Depositors: A Leibovitz
SW 900細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: lung
Tumor Stage: grade IV
Disease: squamous cell carcinoma
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Tumorigenic: Yes
Antigen Expression: Blood type A; Rh+
DNA Profile (STR): Amelogenin: X
CSF1PO: 11
D13S317: 8
SW 900細(xì)胞D16S539: 11
D5S818: 11
D7S820: 11,12
THO1: 8
TPOX: 11
vWA: 16
Cytogenetic Analysis: hypotriploid; modal number = 56. The rate of higher ploidies was 21.8%. Cells in this line have complex karyotypes., Over 30 marker chromosomes were found in 5 metaphase karyotypes. The marker chromosomes, t(1q;10q), t(1q;22q), t(2q;3q), der (3)t(2;3)(p11;q29), der (7)t(7;17) (q36;q21) and about 20 others were common to most cells., Among these der (7) was generally paired. Normal N3, N5, N12, N13, N14 and N17 were absent. The X was single and the Y was not found on fluorescence observation.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1
PGM3, 2
Age: 53 years
Gender: male
Ethnicity: Caucasian
Comments: SW 900細(xì)胞The SW 900 cell line was initiated by A. Leibovitz in January 1975 from biopsy tissue of a squamous cell carcinoma.
The tumor originated in the lung of a 53 year old male Caucasian.
The histopathology of the surgical specimen was determined to be grade IV carcinoma.
A frozen ampule at passage 15 was received at the ATCC in January, 1982.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 100%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
References: 22536: SW 900細(xì)胞Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
22551: Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474
