29SR細胞

生長狀態： 懸浮生長

ATCC Number： CRL-2262?

相關**： 其他**

年限： 11 years

數量： 大量

運輸方式： 凍存運輸

細胞形態： **樣

細胞類型： **母細胞

是否是腫瘤細胞： 1

物種來源： 人

Designations: SR

Depositors: M Beckwith

29SR細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Disease: large cell immunoblastic lymphoma

Derived from metastatic site: pleural effusion

Cell Type: lymphoblast

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Antigen Expression: Hle-1 +; HLA DQ +; HLA DR +; CD25 +; CD19 -; CD20 -; CD21 -; CD22 -; T cell receptor (TCR) -

DNA Profile (STR): 29SR細胞Amelogenin: X,Y

CSF1PO: 14,15

D13S317: 11

D16S539: 11,13

D5S818: 11

D7S820: 9,12

THO1: 6,7

TPOX: 8,11

vWA: 17,18

Age: 11 years

Gender: male

Ethnicity: Caucasian

Comments: SR is a human lymphoma cell line originated in 1983 by Walter J. Urba and Dan L. Longo.

29SR細胞SR is of undetermined cellular origin because it expresses no markers unique to B, T, natural killer (NK) or monocyte-lineages.

Exposure of SR cells to protein kinase C activating phorbol esters such as PMA and PdBu do not induce growth inhibition.

SR cells have been reported to be Epstein-Barr virus genome negative.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 22555: Beckwith M, et al. 29SR細胞Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723